SODIUM BICINCHONINATE,4,4'-DICARBOXY-2,2'-BIQUINOLINE

SODIUM+BICINCHONINATE, that is, diquinoline carboxylic acid, 4,4 '-DICARBOXY-2,2' -BIQUINOLINE refers to 4,4 '-dicarboxyl-2,2' -biquinoline, and both refer to the same compound. Its chemical structure is as follows:
The core of this compound consists of two quinoline rings connected by the 2,2 '-position to form a biquinoline structure. At the 4,4' -position, each is connected with a carboxyl group (-COOH). Due to its acidic nature, it binds to sodium (Na) to form a sodium salt, that is, diquinoline carboxylic acid. In this sodium salt structure, sodium (Na) replaces hydrogen (H) on the carboxyl group to form the -COONa structure.
This structure endows the compound with unique chemical properties and reactivity. In the field of biochemical analysis, it is often used as a protein quantification reagent. Based on its reaction with specific groups in the protein, a product with a specific color is generated, and the protein content is accurately determined by colorimetry. Such a chemical structure is the fundamental reason for its corresponding chemical and biochemical properties.

SODIUM BICINCHONINATE, that is, diquinoline carboxylic acid, 4,4 '-DICARBOXY-2,2' -BIQUINOLINE is a chemical description of its diquinoline carboxylic acid moiety. The main use of this substance is.
In the field of biochemical analysis, it is often used for protein quantification. The principle is to use the complex formed by diquinoline carboxylic acid under the weight of the protein, which can be specific to the protein, and has strong absorption under a specific wave, so as to determine the protein performance. Because of its high sensitivity and high determination, it is widely used in many chambers.
In terms of research, it can be used to determine the content of protein components in the compound. For some protein compounds, it is very important to accurately grasp the content of their active ingredients. The quantitative method of diquinolinecarboxylic acid provides a reliable basis for controlling the amount of protein and ensuring the effectiveness of the product.
In addition, in food, it can also be used to determine the protein content in food. The protein content in the food ingredient list needs to be refined. This method can help food producers to note the ingredients and ensure the benefit of consumers. In addition, SODIUM BICINCHONINATE plays an indispensable role in the field of multi-disciplinary research and quantity control.

Sodium-dioctaninic acid (SODIUM + BICINCHONINATE, 4,4 '-dicarboxyl-2,2' -biquinoline) is a commonly used reagent for biochemical experiments. Its usage is as follows:
This reagent is often used in combination with copper ions to detect protein concentration. In an alkaline environment, the peptide bonds of protein molecules can reduce divalent copper ions to monovalent copper ions, and monovalent copper ions can tightly bind to dioctaninic acid to form a purple complex.
During the experimental operation, the first step is to prepare a series of standard protein solutions with different concentrations, such as 0 μg/mL, 25 μg/mL, 50 μg/mL, 100 μg/mL, 200 μg/mL, 400 μg/mL, etc., as the basis for drawing the standard curve. Then prepare the sample protein solution properly. Take an appropriate amount of the sample and the standard, usually 5-20 μL, and place it in a micro centrifuge tube or a 96-well plate.
Subsequently, the working solution is prepared according to a certain ratio. Generally, the A solution (containing dioctaninic acid and other components) and the B solution (containing copper sulfate and other components) are mixed in a 50:1 ratio. To the tube or well containing the sample and the standard product, add an appropriate amount of working solution, such as 200 μL, and mix gently.
Next, incubate the mixture at 37 ° C for a period of time, about 30 minutes, to promote the reaction to proceed fully. After the reaction is completed, let it cool to room temperature, and use an enzyme scale or spectrophotometer to measure the absorbance value of each tube or well at a wavelength of 562 nm.
Draw a standard curve with the standard protein solution concentration as the abscissa and the corresponding absorbance value as the ordinate. According to the sample absorbance value, find the sample protein concentration on the standard curve. In this way, the protein content of the sample can be accurately determined, which is a common usage of sodium-dioctaninic acid.

SODIUM BICINCHONINATE, that is, diquinoline carboxylic acid, 4,4 '-DICARBOXY - 2,2' -BIQUINOLINE, 4,4 '-dicarboxyl-2,2' -quinoline, this is the most important thing to store.
It should be stored in the dry and dry environment, protected from direct light. Because of light, it is easy to change its properties, such as photochemical reaction, breaking it, and affecting its efficacy.
Furthermore, it is necessary to maintain the dryness of the environment. In the tidal environment, the interaction of moisture is easy to affect, or the reaction such as hydrolysis is induced, causing its composition to change.
The degree is also a factor, usually refrigerated at 2-8 ° C. This temperature can effectively inhibit the reaction rate and maintain its quality. If the temperature is high, the molecular activity is increased, and it is easy to decompose or other adverse reactions; if the temperature is low, it may change the crystal form of the object, and affect its performance.
And it is placed in a sealed container to prevent oxygen, carbon dioxide, etc. from joining in the air. Oxygen may cause oxidation, and carbon dioxide may also be transformed and transformed into it.
In addition, proper storage of parts, such as dryness, temperature, specific temperature and sealed storage, can ensure SODIUM BICINCHONINATE, 4,4 '-DICARBOXY - 2,2' -BIQUINOLINE to maintain good performance and quality.

Sodium Bicinchoninate (4,4 '-Dicarboxy-2,2' -biquinoline), a chemical substance, is commonly used in the room. When using this substance, it is safe to pay attention and must not be ignored.
The first person to pay attention to is the nature of this substance. It may be irritating, and it is difficult to contact the skin and eyes. Therefore, when handling it, it is advisable to prevent the skin from being bound, such as gloves and eyes, to separate the skin from the skin and eyes.
Furthermore, the environment of use is also important. It is advisable to pass through the good place, so that the skin can be scattered and do not gather in the room, to prevent inhalation and harm the body. If you inhale it carelessly, you will move to the empty and fresh place, and you must ask for it.
In addition, there are laws for the storage of this thing. It is appropriate to store it in a place where it is hot, dry, and has a source of ignition and oxidation. It may be flammable and easy to react to other things. It is appropriate to store it, so that it can be fully protected. And properly, so that people can know what it is and prevent it from being used.
If you accidentally drop this thing, it will be cleaned immediately when it is on the table and the ground. According to its nature, it should be removed by appropriate methods, so that it will not be left behind, so as to avoid accidents.
Use Sodium Bicinchoninate to be careful and pay attention to safety, so that you can benefit and protect your health.